Staff profile

Daphne Jackson Fellowship 2009 - 2011

• Biophysical Methods
• Crystallography
• Molecular Biology

Journal Article

• Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix
  Jarrin, M., Kalligeraki, A. A., Uwineza, A., Cawood, C. S., Brown, A. P., Ward, E. N., Le, K., Freitag-Pohl, S., Pohl, E., Kiss, B., Tapodi, A., & Quinlan, R. A. (2023). Independent Membrane Binding Properties of the Caspase Generated Fragments of the Beaded Filament Structural Protein 1 (BFSP1) Involves an Amphipathic Helix. Cells, 12(12), Article 1580. https://doi.org/10.3390/cells12121580
• AmiP from hyperthermophilic Thermus parvatiensis prophage is a thermoactive and ultrathermostable peptidoglycan lytic amidase
  Jasilionis, A., Plotka, M., Wang, L., Dorawa, S., Lange, J., Watzlawick, H., van den Bergh, T., Vroling, B., Altenbuchner, J., Kaczorowska, A., Pohl, E., Kaczorowski, T., Nordberg Karlsson, E., & Freitag‐Pohl, S. (2023). AmiP from hyperthermophilic Thermus parvatiensis prophage is a thermoactive and ultrathermostable peptidoglycan lytic amidase. Protein Science, 32(3), Article e4585. https://doi.org/10.1002/pro.4585
• Cysteine synthase: multiple structures of a key enzyme in cysteine synthesis and a potential drug target for Chagas disease and leishmaniasis
  Sowerby, K., Freitag-Pohl, S., Murillo, A. M., Silber, A. M., & Pohl, E. (2023). Cysteine synthase: multiple structures of a key enzyme in cysteine synthesis and a potential drug target for Chagas disease and leishmaniasis. Acta Crystallographica Section D: Structural Biology, 79(6), 518-530. https://doi.org/10.1107/s2059798323003613
• Derisking the Polymorph Landscape: The Complex Polymorphism of Mexiletine Hydrochloride
  Andrews, J. L., Nilsson Lill, S. O., Freitag-Pohl, S., Apperley, D. C., Yufit, D. S., Batsanov, A. S., Mulvee, M. T., Edkins, K., McCabe, J. F., Berry, D. J., Probert, M. R., & Steed, J. W. (2021). Derisking the Polymorph Landscape: The Complex Polymorphism of Mexiletine Hydrochloride. Crystal Growth and Design, 21(12), 7150-7167. https://doi.org/10.1021/acs.cgd.1c01009
• Flavonoid-based inhibitors of the Phi-class glutathione transferase from black-grass to combat multiple herbicide resistance
  Schwarz, M., Eno, R. F., Freitag-Pohl, S., Coxon, C. R., Straker, H. E., Wortley, D. J., Hughes, D. J., Mitchell, G., Moore, J., Cummins, I., Onkokesung, N., Brazier-Hicks, M., Edwards, R., Pohl, E., & Steel, P. G. (2021). Flavonoid-based inhibitors of the Phi-class glutathione transferase from black-grass to combat multiple herbicide resistance. Organic and Biomolecular Chemistry, 19(42), 9211-9222. https://doi.org/10.1039/d1ob01802g
• Going to extremes - a metagenomic journey into the dark matter of life
  Aevarsson, A., Kaczorowska, A.-K., Adalsteinsson, B. T., Ahlqvist, J., Al-Karadaghi, S., Altenbuchner, J., Arsin, H., Atlasson, U. A., Brandt, D., Cichowicz-Cieslak, M., Cornish, K. A., Courtin, J., Dabrowski, S., Dahle, H., Djeffane, S., Dorawa, S., Dusaucy, J., Enault, F., Fedoy, A.-E., Freitag-Pohl, S., …Zhang, R. (2021). Going to extremes - a metagenomic journey into the dark matter of life. FEMS Microbiology Letters, 368(12), Article fnab067. https://doi.org/10.1093/femsle/fnab067
• Chalcones identify cTXNPx as a potential antileishmanial drug target
  Escrivani, D. O., Charlton, R. L., Caruso, M. B., Burle-Caldas, G. A., Borsodi, M. P. G., Zingali, R. B., Arruda-Costa, N., Palmeira-Mello, M. V., de Jesus, J. B., Souza, A. M., Abrahim-Vieira, B., Freitag-Pohl, S., Pohl, E., Denny, P. W., Rossi-Bergmann, B., & Steel, P. G. (2021). Chalcones identify cTXNPx as a potential antileishmanial drug target. PLoS Neglected Tropical Diseases, 15(11), Article e0009951. https://doi.org/10.1371/journal.pntd.0009951
• Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani
  Zohib, M., Maheshwari, D., Pal, R. K., Freitag-Pohl, S., Biswal, B. K., Pohl, E., & Arora, A. (2020). Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani. Acta Crystallographica Section F: Structural Biology Communications, 76(11), 544-556. https://doi.org/10.1017/s2053230x20013722
• Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS
  Freitag-Pohl, S., Jasilionis, A., Håkansson, M., Svensson, L. A., Kovačič, R., Welin, M., Watzlawick, H., Wang, L., Altenbuchner, J., Płotka, M., Kaczorowska, A. K., Kaczorowski, T., Nordberg Karlsson, E., Al-Karadaghi, S., Walse, B., Aevarsson, A., & Pohl, E. (2019). Crystal structures of the Bacillus subtilis prophage lytic cassette proteins XepA and YomS. Acta Crystallographica Section D: Structural Biology, 75(11), 1028-1039. https://doi.org/10.1107/s2059798319013330
• How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project
  Bruce, D., Cardew, E., Freitag-Pohl, S., & Pohl, E. (2019). How to Stabilize Protein: Stability Screens for Thermal Shift Assays and Nano Differential Scanning Fluorimetry in the Virus-X Project. Journal of Visualized Experiments, 144, Article e58666. https://doi.org/10.3791/58666
• Oxidation of [Li-4\(NBut)(3)S\(2)]: a new route to sulfur triimides
  Fleischer, R., Freitag, S., & Stalke, D. (2018). Oxidation of [Li-4\(NBut)(3)S\(2)]: a new route to sulfur triimides. Dalton Transactions, 193-198. https://doi.org/10.1039/a707085c
• Multiple roles for plant glutathione transferases in xenobiotic detoxification
  Cummins, I., Dixon, D., Freitag-Pohl, S., Skipsey, M., & Edwards, R. (2011). Multiple roles for plant glutathione transferases in xenobiotic detoxification. Drug Metabolism Reviews, 43(2), 266-280. https://doi.org/10.3109/03602532.2011.552910
• Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin
  Le Trong, I., Freitag, S., Klumb, L., Chu, V., Stayton, P., & Stenkamp, R. (2003). Structural studies of hydrogen bonds in the high-affinity streptavidin-biotin complex: mutations of amino acids interacting with the ureido oxygen of biotin. Acta crystallographica. Section D, Biological crystallography, 59(9), 1567-1573. https://doi.org/10.1107/s0907444903014562
• Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system
  Hyre, D., Le Trong, I., Freitag, S., Stenkamp, R., & Stayton, P. (2000). Ser45 plays an important role in managing both the equilibrium and transition state energetics of the streptavidin-biotin system. Protein Science, 9(5), 878-885. https://doi.org/10.1110/ps.9.5.878
• A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway
  Freitag, S., Chu, V., Penzotti, J., Klumb, L., To, R., Hyre, D., Le Trong, I., Lybrand, T., Stenkamp, R., & Stayton, P. (1999). A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway. Proceedings of the National Academy of Sciences, 96(15), 8384-8389. https://doi.org/10.1073/pnas.96.15.8384
• Streptavidin-biotin binding energetics
  Stayton, P., Freitag, S., Klumb, L., Chilkoti, A., Chu, V., Penzotti, J., To, R., Hyre, D., Le Trong, I., Lybrand, T., & Stenkamp, R. (1999). Streptavidin-biotin binding energetics. https://doi.org/10.1016/s1050-3862%2899%2900042-x
• X-ray crystallographic studies of streptavidin mutants binding to biotin
  Freitag, S., Le Trong, I., Klumb, L., Chu, V., Chilkoti, A., Stayton, P., & Stenkamp, R. (1999). X-ray crystallographic studies of streptavidin mutants binding to biotin. https://doi.org/10.1016/s1050-3862%2899%2900048-0
• Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?
  Freitag, S., Le Trong, I., Klumb, L., Stayton, P., & Stenkamp, R. (1999). Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?. Acta crystallographica. Section D, Biological crystallography, 55(6), 1118-1126. https://doi.org/10.1107/s0907444999002322
• Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex
  Freitag, S., Le Trong, I., Chilkoti, A., Klumb, L., Stayton, P., & Stenkamp, R. (1998). Structural studies of binding site tryptophan mutants in the high-affinity streptavidin-biotin complex. Journal of Molecular Biology, 279(1), 211-221. https://doi.org/10.1006/jmbi.1998.1735
• Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system
  Chu, V., Freitag, S., Le Trong, I., Stenkamp, R., & Stayton, P. (1998). Thermodynamic and structural consequences of flexible loop deletion by circular permutation in the streptavidin-biotin system. Protein Science, 7(4), 848-859
• Structural studies of the streptavidin binding loop
  Freitag, S., LeTrong, I., Klumb, L., Stayton, P., & Stenkamp, R. (1997). Structural studies of the streptavidin binding loop. Protein Science, 6(6), 1157-1166. https://doi.org/10.1002/pro.5560060604
• Crystal structure of chloro-dimethylstannyl-diphenylphosphinoyl-ethan-phosphonaciddiisopropyl ester, C22H33ClO4P2Sn
  Freitag, S., & HerbstIrmer, R. (1996). Crystal structure of chloro-dimethylstannyl-diphenylphosphinoyl-ethan-phosphonaciddiisopropyl ester, C22H33ClO4P2Sn. Zeitschrift für Kristallographie - Crystalline Materials, 211(11), 859-860. https://doi.org/10.1524/zkri.1996.211.11.859
• Products of the reactions of 2-diazomalonate and of 2-diazoindanedione with germylenes and stannylenes
  Ossig, G., Meller, A., Freitag, S., Muller, O., Gornitzka, H., & HerbstIrmer, R. (1996). Products of the reactions of 2-diazomalonate and of 2-diazoindanedione with germylenes and stannylenes. Organometallics, 15(1), 408-414. https://doi.org/10.1021/om950599p
• Observation of a Ge-Li bond: Donor-base-stabilized (tris(trimethylsilyl)germyl)lithium
  Freitag, S., HerbstIrmer, R., Lameyer, L., & Stalke, D. (1996). Observation of a Ge-Li bond: Donor-base-stabilized (tris(trimethylsilyl)germyl)lithium. Organometallics, 15(12), 2839-2841. https://doi.org/10.1021/om9600295
• [S(NtBu)(3)](2-) - A cap-shaped dianion, isoelectronic with the sulfite ion and oxidizable to a stable radical anion
  Fleischer, R., Freitag, S., Pauer, F., & Stalke, D. (1996). [S(NtBu)(3)](2-) - A cap-shaped dianion, isoelectronic with the sulfite ion and oxidizable to a stable radical anion. Angewandte Chemie. International edition in English, 35(2), 204-206. https://doi.org/10.1002/anie.199602041
• Monomeric trilithium-tris[tert-butyldimethylsilylamido]phenylsilane, PhSi[NLi(thf)SiMe(2)CMe(3)](3) - Synthesis and crystal structure
  Hemme, I., Klingebiel, U., Freitag, S., & Stalke, D. (1995). Monomeric trilithium-tris[tert-butyldimethylsilylamido]phenylsilane, PhSi[NLi(thf)SiMe(2)CMe(3)](3) - Synthesis and crystal structure. https://doi.org/10.1002/zaac.19956211220
• (N-(1-ADAMANTYL)[(PENTAFLUORO-2-PROPENYL)THIO]AMINO)(FLUORO)BIS[2,4, 6-TRIS(TRIFLUOROMETHYL)PHENYL]TIN AT 153 K
  FREITAG, S., Herbstirmer, R., Ahlemann, J., & Roesky, H. (1995). (N-(1-ADAMANTYL)[(PENTAFLUORO-2-PROPENYL)THIO]AMINO)(FLUORO)BIS[2,4, 6-TRIS(TRIFLUOROMETHYL)PHENYL]TIN AT 153 K. Acta crystallographica. Section C, Crystal structure communications, 51(4), 631-633. https://doi.org/10.1107/s0108270194011996
• SYNTHESIS OF THE NEW SILANEDIYLDIPHOSPHINITE TBU(2)SI(OPPH(2))(2) AND ITS REACTIONS WITH THE NORBORNADIENE COMPLEXES C(7)H(8)M(CO)(4) (M=CR, MO, W) - CRYSTAL-STRUCTURES OF CIS-M(CO)(4),[TBU(2)SI(OPPH(2))(2)] (M=CR, MO)
  Voelker, H., FREITAG, S., Pieper, U., & Roesky, H. (1995). SYNTHESIS OF THE NEW SILANEDIYLDIPHOSPHINITE TBU(2)SI(OPPH(2))(2) AND ITS REACTIONS WITH THE NORBORNADIENE COMPLEXES C(7)H(8)M(CO)(4) (M=CR, MO, W) - CRYSTAL-STRUCTURES OF CIS-M(CO)(4),[TBU(2)SI(OPPH(2))(2)] (M=CR, MO). https://doi.org/10.1002/zaac.19956210433
• TETRAISOPROPYL 2-[(BROMO)(CHLORO)(METHYL)STANNYL]-1,1-ETHANEDIPHOSPHONATE AT 153K
  FREITAG, S., Herbstirmer, R., Richter, F., & Weichmann, H. (1994). TETRAISOPROPYL 2-[(BROMO)(CHLORO)(METHYL)STANNYL]-1,1-ETHANEDIPHOSPHONATE AT 153K. Acta crystallographica. Section C, Crystal structure communications, 50(10), 1588-1590. https://doi.org/10.1107/s0108270194004105
• DILITHIUM N,N'-BIS(TRIMETHYLSILYL)HYDRAZIDE AND A HYDROLYSIS PRODUCT - SIDE-ON-COORDINATED AND END-ON-COORDINATED LI CATIONS TO N2(2-) AND O2(2-)
  Drost, C., Jager, C., FREITAG, S., Klingebiel, U., Noltemeyer, M., & Sheldrick, G. (1994). DILITHIUM N,N'-BIS(TRIMETHYLSILYL)HYDRAZIDE AND A HYDROLYSIS PRODUCT - SIDE-ON-COORDINATED AND END-ON-COORDINATED LI CATIONS TO N2(2-) AND O2(2-). https://doi.org/10.1002/cber.19941270510
• REACTIONS OF A FREE STANNAIMINE AND OF BASE-STABILIZED STANNYLENES
  Ossig, G., Meller, A., FREITAG, S., Herbstirmer, R., & Sheldrick, G. (1993). REACTIONS OF A FREE STANNAIMINE AND OF BASE-STABILIZED STANNYLENES. https://doi.org/10.1002/cber.19931261013
• IMINOAMINOSULFINATES - SYNTHESIS, CRYSTAL-STRUCTURES, AND REARRANGEMENT MONITORED BY LI-7 SOLID-STATE NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY
  FREITAG, S., Kolodziejski, W., Pauer, F., & Stalke, D. (1993). IMINOAMINOSULFINATES - SYNTHESIS, CRYSTAL-STRUCTURES, AND REARRANGEMENT MONITORED BY LI-7 SOLID-STATE NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPY. Dalton Transactions, 3479-3488. https://doi.org/10.1039/dt9930003479
• SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF A STANNAIMINE
  Ossig, G., Meller, A., FREITAG, S., & Herbstirmer, R. (1993). SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF A STANNAIMINE. Journal of the Chemical Society. Chemical communications, 497-499. https://doi.org/10.1039/c39930000497
• SYNTHESIS AND CRYSTAL-STRUCTURE OF THE SPIROCYCLE [(I-PR)2P(S)NSIME3]2SNCL2
  Raubold, T., FREITAG, S., Herbstirmer, R., & Roesky, H. (1993). SYNTHESIS AND CRYSTAL-STRUCTURE OF THE SPIROCYCLE [(I-PR)2P(S)NSIME3]2SNCL2. https://doi.org/10.1002/zaac.19936190524
• INVESTIGATIONS OF THE STRUCTURE AND REACTIVITY OF A STANNAKETENIMINE
  Grutzmacher, H., FREITAG, S., Herbstirmer, R., & Sheldrick, G. (1992). INVESTIGATIONS OF THE STRUCTURE AND REACTIVITY OF A STANNAKETENIMINE. Angewandte Chemie. International edition in English, 31(4), 437-438. https://doi.org/10.1002/anie.199204371
• REACTIONS OF GERMYLENES WITH ACID AZIDES
  Meller, A., Ossig, G., Maringgele, W., Noltemeyer, M., Stalke, D., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1992). REACTIONS OF GERMYLENES WITH ACID AZIDES
• [3 + 1] CYCLOADDITION - REACTION OF DICHLOROGERMYLENE WITH HEXAFLUORO-2-PROPANETHIONE 1-ADAMANTYLIMIDE
  May, A., Roesky, H., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1992). [3 + 1] CYCLOADDITION - REACTION OF DICHLOROGERMYLENE WITH HEXAFLUORO-2-PROPANETHIONE 1-ADAMANTYLIMIDE. Organometallics, 11(1), 15-16. https://doi.org/10.1021/om00037a008
• SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF GERMAIMINES
  Meller, A., Ossig, G., Maringgele, W., Stalke, D., Herbstirmer, R., FREITAG, S., & Sheldrick, G. (1991). SYNTHESIS AND X-RAY CRYSTAL-STRUCTURE OF GERMAIMINES. Journal of the Chemical Society. Chemical communications, 1123-1124. https://doi.org/10.1039/c39910001123